NANOVI

Repair your cells in 15 minutes

NANOVI

Visualizing the effects of the NanoVi on cells

Cell damage is natural and inevitable. While the NanoVi cannot prevent cell damage, it can help cells repair cellular damage. The following scenarios illustrate the NanoVi’s influence on cellular damage & cell restoration processes:

In scenario one, free radicals attack a cell, causing cell damage to proteins, mitochondria, DNA, and the cell membrane. Repair is initiated through the use of ROS signaling, the body’s mechanism for triggering repair. Once ROS signals are emitted and received, the signaling molecules are recycled back into metabolic processes as free radicals.In scenario one, there is a great deal of free radical damage without any intervening, supplementary antioxidant protection: The body is left to fend off free radical damage without any exogenous support.

In scenario two, we see a cell that is healthy with adequate fitness and physiological protections, including from antioxidants. By taking preventative measures, especially when supplementing with high antioxidant foods and supplements, cellular health is maintained with a better balance of free radicals to ROS signals. In this example, the amount of ROS and ROS signals are nearly identical.In scenario three, we see a cell that is the beneficiary of both additional endogenous as well as exogenous ROS signals and antioxidants (from diet). By lessening the amount of toxic ROS via antioxidant intake and adding the bio-identical signals provided by the NanoVi, we can augment the body’s natural cell repair capacities.

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a deeper dive into protein folding

What are ROS and how are they generated?

Reactive oxygen species (ROS) play a multitude of signaling roles in different organisms from bacteria to mammalian cells. ROS were initially thought to be toxic byproducts of aerobic metabolism, but have now been acknowledged as central players in the complex signaling network of cells. ROS arise from the one-electron reduction of molecular oxygen. Intracellular ROS exist primarily in three forms: superoxide anions (O2−), hydrogen peroxide (H2O2) and hydroxyl radicals (OH−). The superoxide anion contains an unpaired electron that imparts high reactivity and necessitates a rapid reduction to H2O2 by the antioxidant enzyme superoxide dismutase (SOD). H2O2 can be further reduced to H2O and O2 by various cellular antioxidants. Although ROS were originally thought to be merely a harmful byproduct of metabolism, accumulating evidence demonstrates a role for ROS in cell fate signaling. H2O2 is thought to be the main ROS species involved with intracellular signaling, and in specific contexts can act directly as a second messenger, integrating environmental cues and passing them to downstream signal transduction cascades. This is due mostly to the longer half-life of H2O2 and its ability to diffuse easily through membranes relative to other types of ROS. Under normal physiological conditions, the generation of ROS is tightly regulated by the ROS scavenging system. ROS scavengers are antioxidant enzymes that can neutralize ROS by directly reacting with and accepting electrons from ROS. When ROS production outpaces ROS scavenging, an excessive accumulation of ROS occurs, leading to oxidative stress and producing adverse effects on multiple cellular components, including proteins, lipids and nucleotides. To counteract this, the cell contains multiple types of antioxidants that are specific to different species of ROS, which helps to prevent pathological levels of ROS and to repair oxidative damage to cellular components. These include superoxide dismutase (SOD), catalase, peroxiredoxins (PRX), thioredoxin (TRX), glutathione peroxidase (GPX) and glutathione reductase (GR). Glutathione, a tripeptide, is one of the most abundant antioxidants synthesized by the cell. Oxidized proteins and H2O2 are reduced by glutathione through the glutaredoxin and thioredoxin system. Other key antioxidants include SOD and catalase, which reduce O2− and H2O2, respectively. The subcellular localization of antioxidants at areas of high ROS generation, such as within the mitochondria, may further enhance the efficiency of ROS scavenging.

Origins of the reactive oxygen species (ROS) network

It is easy to imagine how cells had to acquire different antioxidants and ROS scavenging/detoxifying enzymes during evolution to cope with the increased levels of atmospheric oxygen that accompanied the appearance of oxygen-evolving microorganisms on Earth billions of years ago. It is nevertheless harder to imagine how ROS with their toxic potential can play such a current key signaling role in cells. When considering the evolution of ROS as important signaling molecules we can assume that once cells learned to deal with ROS toxicity, they were able to utilize ROS for signaling purposes. Moreover, we can also assume that there are numerous advantages for using ROS as signaling molecules. What is it therefore that makes ROS such good signaling molecules (after all if cells evolved to use them as such they must have their advantages)? Several possible advantages come to mind when considering the use of ROS as signaling molecules. These include the capacity of the cell to rapidly produce and scavenge different forms of ROS in a simultaneous manner, enabling rapid and dynamic changes in ROS levels (caused by simply tilting the balance between cellular production and scavenging rates). Another advantage could be a tight control over the subcellular localization of ROS signals in cells. If we assume a significant capacity of cells to detoxify/ scavenge or buffer ROS throughout the cell, then local increases in ROS production can be limited to particular locations of the cell, such as a certain membrane patch, or organelle, making the spatial control of ROS accumulation highly specific. Another advantage of ROS is that they could be used as rapid long distance auto-propagating signals transferred throughout the body. Each individual cell along the path of the signal could activate its own ROS producing mechanism(s) in an autonomous manner carrying a ROS signal over long distances. An additional signaling advantage of ROS is that different forms of ROS exist, with significantly different molecular properties. For example, superoxide is a charged molecule under most physiological conditions and could not passively transfer across a membrane. By contrast, superoxide could be easily converted into hydrogen peroxide (H2O2) that readily transfers across membranes passively or through water channels. Superoxide and H2O2 can also mediate the formation of lipid peroxides that would be membrane soluble. Thus, ROS have the advantage of being versatile signaling molecules with regard to their properties and mobility within cells. Moreover, as part of a cellular signaling network, ROS could be integrated with several different signaling pathways. Links with calcium and protein phosphorylation networks have been extensively studied, for example in the case of the ROS-generating respiratory burst oxidase (RBOH) NADPH oxidase proteins that contain an EF-calcium binding as well as phosphorylation domain(s). In addition, ROS levels are linked with cellular redox networks, for example through thioredoxins, peroxiredoxins, glutaredoxins and/or NADPH. Another key signaling advantage of ROS is their tight link to cellular homeostasis and metabolism. Almost any change in cellular homeostasis could lead to a change in the steady-state level of ROS in a particular compartment(s). It is easy to envision how a tight link between metabolism and ROS levels would make ROS good signals to monitor changes in cellular metabolism. It is also possible that this was the initial evolutionary advantage to using ROS as signaling molecules, an advantage that led to further and future use of ROS to signal and control many different biological processes . Because different organisms generate ROS at different levels and could leak or actively transport ROS such as H2O2 into their environment, it is possible that another advantage of ROS as signaling molecules in early stages of evolution was the sensing and/or communication between different organisms. Thus, the early need to sense and control internal (metabolic), as well as external (environmental/ other organisms/other cells), sources of ROS might have contributed to the evolution of ROS as key signaling molecules.

Sources of ROS

The electron transport chain, a component of mitochondria that is responsible for mitochondrial respiration, is the main source of ROS within the cell. The primary role of the electron transport chain is to generate the proton motive force, which leads to ATP production through ATP synthase in a process known as oxidative phosphorylation. However, ∼0.1-0.2% of O2 consumed by mitochondria is thought to form ROS through the premature electron flow to O2, mainly through electron transport chain complexes I and III. The precise proportion of ROS generated from mitochondrial respiration can differ greatly depending on the cell type, environment and, ultimately, the activity of mitochondria. Thus, another method of cellular regulation of ROS levels is through control of mitochondrial function and the regulation of metabolic pathways. Specifically, reduced ROS levels can be achieved by diverting substrates away from oxidative phosphorylation to decrease the rate of mitochondrial respiration. In addition, ROS levels can also be minimized by diverting metabolic substrates through processes that regenerate oxidized glutathione, such as the pentose phosphate pathway. Another major source of ROS is the membrane-bound protein NADPH oxidase (NOX), which consumes NADPH to generate O2− and, subsequently, H2O2. ROS produced by NOX have been shown to act as anti-microbial molecules and also to enhance growth factor signaling.

NanoVi Technology in Relation to Oxygen and Antioxidant Therapies

Oxygen is essential to the chemical process of cell energy production but this process also produces reactive oxygen species (ROS). For the most part ROS are damaging free radicals but they also function as signaling molecules or ‘second messengers.’ When acting as second messengers, ROS play a key role in initiating the body’s repair mechanisms. NanoVi’s bio-identical signaling technology relies on an ROS-specific signal to influence cellular repair mechanisms and consequently protect and improve cellular activity. NanoVi is the first and only device to emit a verifiable ROS-specific signal without the creation or use of harmful ROS.

Oxygen therapies

Input: oxygen
Reaction: produce cell energy, but generate ROS

Oxygen therapies have been used for centuries to deliver more oxygen to the cells to compensate for insufficient cell energy production. When there is damage affecting oxygen utilization in the cells, oxygen exchange in tissue, or oxygen transport, additional oxygen is often supplied. Oxygen therapies, like hyperbaric chambers or supplemental oxygen, including exercise with oxygen therapy EWOT, increase the percent of oxygen above normal levels. Oxygen therapies help generate cell energy but produce additional ROS. Most of these ROS act as free radicals and cause cellular damage, which the body must repair to maintain health.

Antioxidant therapies

Input: antioxidants
Reaction: neutralize free radicals, but eliminate signaling molecules

Antioxidants have been administered for more than half a century to address disease and age-accelerating free radicals. Antioxidants are used in chemical processes that eliminate ROS molecules. Most ROS act as free radicals and damage other cellular components.To avoid this damage, ROS must be eliminated as early as possible. Antioxidant therapies include oral or intravenous delivery of molecules to the cells to inhibit the oxidation caused by ROS. Simply put, the anti-oxidants “catch” the ROS. Unfortunately the anti-oxidants eliminate not only the ROS that would act as free radicals, but also the ROS that would act as signaling molecules. Therefore excess anti-oxidants handicaps cell repair functions because it eliminates important ROS signaling molecules. Excessive use of antioxidants can be too much of a good thing, detroying the ROS signaling molecules necessary for optimal biologic function.

Bio-identical signaling therapy

Input: ROS-specific signal
Reaction: increase signal-initiated repair mechanisms

Over the last 15 years scientists have confirmed that ROS-specific signaling molecules act as ‘second messengers’ and are essential in triggering certain types of cellular repair. Lifestyle, environment, and aging accelerate the need for repair as damage accumulates. A single DNA, for example, is damaged more than 700,000 times per day and needs to be repaired as fast as possible. Bio-identical signaling therapy delivers the ROS-specific signal needed to trigger cellular repair. Helping initiate the body’s natural repair mechanisms improves cellular activity and the spectrum of free radical-damaged cellular functions is addressed. Bio-identical signaling does not generate any other molecules, suppress other functions, or interfere with oxygen or anti-oxidant therapies. Quite the contrary, it offsets the negative aspects of oxygen therapies by promoting cellular repair. It also overcomes the problem of impeding crucial second messengers with anti-oxidants because the necessary signal is delivered by the technology.

How does NanoVi™ make a signal that is bio-identical to what our bodies make?

The simple answer is that the NanoVi creates a specific electromagnetic wave that has precisely the same wavelength as the ROS signals produced in our cells, which is then imprinted into water. This signal is then inhaled and delivered to the body via water vapor where it initiates cellular repair mechanisms. NanoVi’s “bio-identical signal” assists the multi-step process of reinstalling protein functions that are essential to cellular activity.

NANOVI & Protein Folding

More than 99% of our body’s molecules are water. The DNA and proteins, which are the second most abundant compound, are embedded in this water. Hundreds of thousands of different proteins execute every process in your body. Tens of thousands are found in each cell. Folded proteins are essential for cellular activity that keeps us vital, healthy and alive. Unfortunately, folded proteins and the DNA are constantly damaged by free radicals during oxidative stress. When unfolded, they lose their function. This reduces cellular activity. The results are loss of performance, aging and, in a worst-case scenario, chronic disease. Fortunately, repair and regeneration are possible and occur when proteins fold and refold. Always, along with free radicals, signaling molecules known as excited or activated oxygen are generated and emit a specific electromagnetic energy. This energy is absorbed and transferred by water, forming layers of ordered water on its contact surfaces. These layers are also called exclusion zones or EZ-water. It is more densely packed and has a higher energy state. They provide the energy required for proteins to fold. When enough layers surround a protein, the folding happens spontaneously and the protein start functioning. This essential biological process is assisted by the patented NanoVi technology. The NanoVi device uses the same signal that is emitted into water droplets in an airstream, leading to the formation of ordered-water vapor. Universities and test-facilities verified this signal’s quality and intensity as bio-identical, and that the vapor contains EZ water. Inhaling NanoVi’s ordered-water vapor affects the entire body. Thanks to the unique properties of water, NanoVi’s specific energy is transferred from vapor droplets to cells and ultimately to proteins. Here, NanoVi provides much-needed energy to the protein folding process. Not only do DNA molecules repair faster, but all cellular activities improve with proper protein function. By assisting their folding, functions are not lost, but are reinstalled.

Who needs to worry about free radicals and oxidative stress?

Everyone - there's no avoiding it. Free radicals damage your cells each day. Damaged cells lead to aging and poor health. So if you want to strengthen your immune system, increase your vitality, and slow the aging process, you'll want to understand how excess free radicals and oxidative stress can harm your body. Combating free radicals and oxidative stress is a great way to increase your vitality, strengthen your immune system and slow the aging process. All cells convert glucose and oxygen into energy. This energy generation process is called “cell metabolism” (oxidative phosphorylation) and its functional operation is essential for cell fitness. Because metabolic processes are always producing ROS at differing rates, cellular damage almost always outpaces cellular repair. Antioxidants can help slow or prevent damage by neutralizing ROS, but they are not sufficient alone to repair cell and DNA damage. It is simply not possible to repair all of the accumulated cellular damage that occurs each day. By producing precisely the same signal as the endogenous ROS do, the NanoVi™ augments your body's natural repair mechanisms. The NanoVi helps non-invasively and without chemicals. Nanovi therapy benefits include:

  • Protection Against & Repair of Free Radical Damage
  • Protection From Adverse Effects of Aging
  • Balancing of Immune System Function
  • Enhanced Utilization of Oxygen & Nutrients
  • Upregulation of Cellular Activities (Energy Production & Detoxification)
  • Enhanced Workout Performance and Recovery
  • Faster Cellular Regeneration
  • Enhanced Protein Repair
  • Inflammatory Support